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Objective@#Here we aimed to investigate the difference in clinical characteristics and outcomes between pediatric and adult patients with COVID-19.@*Methods@#A total of 333 consecutive patients with laboratory-confirmed SARS-CoV-2 infection treated in the departments of Internal medicine of Shenzhen Third People's Hospital from January 11 @*Results@#Compared with adult patients, pediatric patients had a shorter time of symptom onset to hospitalization than adults [median time, 1 ( @*Conclusion@#Pediatric patients with COVID-19 had milder or less clinical symptoms, less evident pulmonary imaging changes, better prognosis, and shorter length of hospital stay.
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Child , Female , Humans , Male , COVID-19/therapy , China/epidemiology , Hospitalization , Length of Stay , Retrospective Studies , SARS-CoV-2 , Treatment OutcomeABSTRACT
Objective At present, the model of pulmonary blast injury is characterized by complexity, a certain degree of safety risks,and difficulty in obtaining the materials.This article aimed to establish a simple, safe and reusable primary pulmonary blast injury model in rats. Methods A total of 16 SD rats were randomly divided into control group and pulmonary blast injury group. In rats of control group, only ketamine(120 mg/kg)was injected intraperitoneally and hair was removed from chest.In pulmonary blast injury group,after the rats were fully anesthetized and depilated on chest,they were placed in a reducing T-pipe with only chest fully exposed,and the pneumatic simulated blasting device was adjusted to 0.4 Mpa.Taking locations about 2.5 cm from the intersection points of the median line of the sternum with left and right anterior axillary lines as targets,explosion were carried out respectively,fol-lowing the sequence of right chest 2 min prior to left chest.Lung pathology,lung water content,arterial blood gas analysis, and inter-leukin-6(IL-6)changes were observed. Results No obvious dam-age was found as to the appearance of chest in all rats after blast inju-ry.Transient purpura appeared in 37.5%(3/8)of the rats within 5 min after injury and disappeared within 1 h.On the spot,62.5%(5/8)of the rats presented 10-20 s of brief apnea and gradually recov-ered 6 h later.The lung tissue W/D,serum IL-6 level and lung tissue IL-6 level in pulmonary blast injury group were significantly greater than those in control group[(4.97±0.37)vs(4.62±0.07),(68.13± 59.40)vs(16.27 ±5.08)pg/mL,(487.04 ±30.57)vs(172.74 ±35.02)pg/mL],representing statistically significant difference(P<0.05).PaO2level in pulmonary blast injury group was significantly lower than that of control group,and the difference was statistically significant(P<0.05).After pulmonary blast injury, punctate and flaky hemorrhages were observed in the lesions of lung tissues,showing red hepatic changes.Lung interstitium was obviously thickened and alveoli collapsed under microscope.Erythrocytes and edema fluid in lung interstitium and alveoli exuded.Gas embolism in intersti-tial blood vessels was observed under microscope. Conclusion The lungs of rats after blast through simple T-pipe is typical of prima-ry pulmonary blast injury in pathological and pathophysiological manifestations, which proves the convenience and reliability of the modeling method.
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Objective By taking rehabilitation as the control, to observe the effect of abdominal and wrist-ankle acupuncture plus rehabilitation in restoring the motor and neurological function in hemiplegia due to ischemic cerebral stroke, and to optimize the treatment protocol. Method By adopting a randomized controlled clinical design, a hundred patients diagnosed with hemiplegia after ischemic cerebral stroke were randomized into a treatment group (50 cases) and a control group (50 cases) regarding their visiting sequence. The patients all received basic symptomatic treatment to control blood pressure, improve cerebral circulation, stabilize vital signs and prevent complications. In addition, the treatment group received abdominal and wrist-ankle acupuncture plus kinesiotherapy; the control group received rehabilitation training. The two groups were evaluated for therapeutic efficacy after 2 courses of treatment. Before and after the intervention, the recruited patients were tested by Fugl-Meyer Assessment (FMA), China Stroke Scale (CSS) and Berg Balance Scale (BBS), and the level of homocysteine (Hcy) was also detected. Result The FMA, CSS and BBS scores all showed significant changes after the intervention in the two groups (P<0.05); after the treatment, the FMA, CSS and BSS scores in the treatment group were significantly different from those in the control group (P<0.05), and there were significant between-group differences in comparing the score differences of FMA,CSS and BBS (P<0.05). The two groups both showed decrease of Hcy, (11.68±4.53)μmol/L in the treatment group versus (13.53±4.01)μmol/L in the control group, and the between-group difference was statistically significant (P<0.05). Conclusion Compared to rehabilitation alone, abdominal and wrist-ankle acupuncture plus kinesiotherapy can produce a more significant efficacy in treating hemiplegia due to ischemic cerebral stroke.
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To evaluate the clinical value of gene chip technology (GCT) in detecting the mycoba-cteria,isoniazid and rifampin resistance of patients diagnosed tuberculous empyema.The 182 patients who met the inclusion criteria were enrolled to this study from January 2011 to December 2015,whose pus mycobacterial species were detected by GCT and MGIT,the simultaneous and sensitivity of them were compared.Meanwhile,36 patients diagnosed tuberculous empyema were selected to detect isoniazid and rifampin resistance.The simultaneous and sensitivity of GCT were evaluated base on the standard of MGIT.The 135 patients were diagnosed by tuberculous empyema.The specificity of GCT was same to MGIT (95.7％),the the sensitivity was 48.9％ (66/135)in GCT,26.7％ in MGIT,there was significant difference between them (x2=80.5,P＜ 0.05).The sensitivity,specificity and coincidence rate of GCT in rifampin resistance were 100％,the sensitivity,specificityand coincidence rate in INH were 50.0％(1/2),97.1％(33/34) and 94.4％.Gene chip technology for detection of mycobacteria has high sensitivity and specificity,which can identify non-tuberculous mycobacteria quickly.And it can also effectively detect the resistance of Mycobacterium tuberculosis to isoniazid and rifampicin.It has important significance in early diagnosis and treatment of tuberculous empyema.
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Objective To investigate the clinical characteristics and related risk factors of infarction secondary to severe traumatic brain injury.Methods 480 traumatic brain injury patients were chosen.Depending on the occurrence of cerebral infarction,patients were divided into TCI groups and non-TCI group,clinical symptoms and signs of TCI group were observed,and its related risk factors was analyzed.Results In 480 cases patients,there were 30 cases of patients with traumatic brain injury secondary to cerebral infarction,the rate was 6.25%.Clinical manifestations included unilateral limb motor and sensory dysfunction,visual dysfunction,language dysfunction,dizziness,headache.10 cases Prognosis were good,6 cases were mild disability,3 cases were severe disability,1 case was plant survival,10 patients died.Univariate analysis showed that the rates of aged ≥50 years,GCS score ＜ 8 points,hernia,hypotension,subarachnoid hemorrhage,large doses of non-dehydrating agent in the TCI group were higher than those of non-TCI group,the differences were statistically significant (x2 =12.311 3,14.725 4,19.867 8,5.296 9,9.242 6,11.713 6,all P ＜ 0.05).Logistic multivariate analysis showed that age ≥50 years,GCS score ＜ 8 points,hernia,cerebral hypotension were important risk factors.Conclusion Brain injury patients with cerebral infarction secondary to clinical manifestations have some characteristics.Age ≥50 years,GCS score ＜ 8 points,hernia,hypotension are important risk factors.
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<p><b>OBJECTIVE</b>To develop a sensitive and specific microarray for detecting mutations of HBV pre-core/core and basic core promoter regions in the clinic.</p><p><b>METHODS</b>Site-specific oligonucleotide probes were designed and immobilized to microarray slides and hybridized to HBV gene fragments amplified with specific biotin-labeled primer using asymmetrical PCR. The specificity and sensitivity of the method were estimated. And the microarray was applied to detect 138 clinical serum samples with HBV-DNA.</p><p><b>RESULTS</b>The mutations of HBV pre-core/core and basic core promoter regions can be specifically detected using the microarray, and the sensitivity was 1 x 10(1) copies/microl. Among 138 samples, 40 samples had T1762/ A1764 mutation, 11 samples had C1814 mutation, and 16 samples had A1896 mutation. The A1896 mutation rate in high HBV-DNA load group was significantly higher than that in low HBV-DNA load group (P < 0.01).</p><p><b>CONCLUSION</b>An DNA microarray assay was successfully established to detect the mutations in HBV pre-core/core and basic core promoter regions. The A1896 mutation in pre-core/core region maybe involve in duplication of HBV.</p>
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Adult , Female , Humans , Male , Middle Aged , Young Adult , Hepatitis B , Virology , Hepatitis B virus , Genetics , Mutation , Oligonucleotide Array Sequence Analysis , Methods , Promoter Regions, Genetic , Viral Core Proteins , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To study the reliability and feasibility of video-assisted thoracoscopic surgery (VATS) for radical resection of early-stage non-small cell lung cancer (NSCLC).</p><p><b>METHODS</b>Fifty-four patients with NSCLC treated between Jan. 2007 and Jun. 2010 at our institution were divided into VATS group (n=23) and video-assisted mini thoracotomy (VAMT) group (n=31). The operative time, intraoperative blood loss, number of dissected nodes, pleural effusion drainage, postoperative hospital stay, and visual analogue scales (VAS) were compared between the two groups.</p><p><b>RESULTS</b>No deaths or serious complications occurred perioperatively in the two groups. The operative time, intraoperative blood loss, number of dissected lymph nodes or pleural effusion drainage were all comparable between the two groups, but compared with VAMT, VATS was associated with significantly shortened postoperative hospital stay (10.54±1.21 days vs 7.92±0.86 days, P<0.05) and lowered VAS scores (4.26±1.28 vs 2.37±0.25, P<0.05).</p><p><b>CONCLUSION</b>VATS for pulmonary lobe resection with systematic node dissection is a feasible approach to the management of early-stage NSCLC.</p>
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Aged , Female , Humans , Male , Middle Aged , Carcinoma, Non-Small-Cell Lung , General Surgery , Feasibility Studies , Lung Neoplasms , General Surgery , Lymph Node Excision , Thoracic Surgery, Video-AssistedABSTRACT
<p><b>OBJECTIVE</b>To explore the best method for prevention and treatment of thrombosis and its mechanism.</p><p><b>METHODS</b>Forty SD rats were randomly divided into a blank group, a model group, an electroacupuncture group and a crude herb moxibustion group. In the electroacupuncture group and the crude herb moxibustion group. "Zusanli" (ST 36), "Sanyinjiao" (SP 6), "Pishu" (BL 20) and "Geshu" (BL 17) were punctured or moxibusted for 2 weeks, then pre-thrombosis model was induced by Adrenalin Hydrochloride and ice water method in the model group, the electroacupuncture group and the crude herb moxibustion group, respectively. Molecular markers in venous blood after the model made in 18 hours were detected.</p><p><b>RESULTS</b>Act: vaty of tissue-type plasminogen activator (t-PA) and content of alpha-granule membrane protein (GMP-140) decreased and content of nitrogen monoxidum (NO) increased after electroacupuncture or crude herb moxibustion. The levels of t-PA and GMP-140 in the model group were higher than those in the electroacupuncture group, the crude herb moxibustion group and the blank group (all P < 0.05), and the content of NO in the model group was lower than those in the electroacupuncture group, the crude herb moxibustion group and the blank group (all P < 0.05), while there was no significant difference in t- PA, GMP-140 and NO among the crude herb moxibustion group, electroacupuncture group and blank group (all P > 0.05).</p><p><b>CONCLUSION</b>Electroacupuncture and crude herb moxibustion can significantly change the contents of t-PA, GMP-140 and NO and there was no significant difference between the two therapies.</p>
Subject(s)
Animals , Female , Humans , Male , Rats , Acupuncture Therapy , Disease Models, Animal , Moxibustion , Nitric Oxide , Blood , P-Selectin , Blood , Random Allocation , Rats, Sprague-Dawley , Thrombosis , Blood , Pathology , Therapeutics , Tissue Plasminogen Activator , BloodABSTRACT
<p><b>OBJECTIVE</b>To provide scientific methods for quality criterion by studying the chemical components of essential oil from Baeckea frutescens.</p><p><b>METHOD</b>The chemical components of essential oil from B. frutescens were identified by GC-MS-DS, TLC and capillary GC. The relative contents of main components were determined by area normalization.</p><p><b>RESULT</b>More than 50 peaks were separated, and 38 components were identified, which accounted for over 94% of the total GC peaks areas of the essential oil. The methods for quality evaluation of essential oil from B. frutescens by TLC and capillary GC were established.</p><p><b>CONCLUSION</b>The chemical components of essential oil from B. frutescens collected from different habitats and collecting periods have common characteristics as well as differences. Some components, such as linalool, can be used as a standard and chromatography fingerprint to analyze the quality of essential oil from B. frutescens.</p>
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Bridged Bicyclo Compounds , China , Cyclohexanols , Ecosystem , Monoterpenes , Myrtaceae , Chemistry , Oils, Volatile , Chemistry , Plant Leaves , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , Chemistry , Quality Control , SeasonsABSTRACT
<p><b>OBJECTIVE</b>To understand the characteristics of human calicivirus (HuCV) infection in infants with diarrhea in Guangzhou city and to study genotype of the virus.</p><p><b>METHODS</b>The authors collected fecal specimens from 22 children with acute nonbacterial gastroenteritis from November to December, 2001. HuCV was detected from the specimens by RT-PCR. The PCR products were cloned into the PMD18-T cloning vector and sequenced.</p><p><b>RESULTS</b>HCV was detected from the specimens of 2 cases (9%, 2/22). The nucleotide sequence analysis revealed that the virus strains belonged to genotype 2 of Norwalk-like viruses.</p><p><b>CONCLUSION</b>HuCV is one of the pathogens causing diarrhea in infants and young children in Guangzhou area. HuCV infection occurred sporadically in autumn and winter.</p>
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Humans , Infant , Base Sequence , Caliciviridae , Genetics , Caliciviridae Infections , Virology , China , DNA, Viral , Chemistry , Genetics , Diarrhea, Infantile , Dysentery , Feces , Virology , Genotype , Molecular Sequence Data , Phylogeny , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Sequence Homology, Nucleic AcidABSTRACT
Objective To explore the epidemics of influenza viruses in Guangzhou area from 1998 to 2003. Methods The specimens for viral isolation were taken with swabs from children's throats and the material was inoculated into the MDCK cells and were incubated at 33 ℃ The supernatant of MDCK cells culture was tested with hemagglutination test. Results Influenza viruses were isolated from 264 of 3444 children; total positive rate of influenza virus isolation was 7.6%. The positive rate of influenza viruses was 16.8% in 1998; the prevailing strain of influenza viruses was H3N2. The influenza viruses isolation rate was 7.4% in 1999;the positive rate was 8.4% ; HIN1 occurred in 2000, the positive rate was 3.8%. H3N2 did not occur in 2001; the positive rate was 7.3% ; influenza B viruses was the prevailing strain in 2002; the positive rate was 1.7% in 2003. Influenza B viruses was Yamagata like strain from 1998 to 2001, Victoria like strain from 2002 to 2003. H9N2 avian influenza virus was isolated from a child. Conclusions Influenza was prevalent in Guangzhou in 1998, but not prevalent from 1999 to 2003. Most of influenza B viruses were Yamagata strain. There were cases avian influenza caused by H9N2 in 1999.